20 Sterile Processing Quiz Questions and Answers

Sterile processing is a vital healthcare function that ensures medical instruments, devices, and supplies are thoroughly cleaned, sterilized, and prepared to prevent infections and maintain patient safety. This process typically involves several key stages:

Decontamination: Instruments are initially cleaned to remove visible soil, blood, and organic matter using enzymatic detergents, manual scrubbing, or automated washers to reduce microbial load.

Preparation and Packaging: Items are inspected for damage, assembled into sets, and wrapped or placed in containers that allow sterilization while maintaining sterility post-process.

Sterilization: High-level methods are applied, such as steam under pressure (autoclaving), ethylene oxide gas, hydrogen peroxide plasma, or low-temperature systems, to destroy all microorganisms, including spores.

Storage and Distribution: Sterile items are stored in controlled environments to prevent contamination and distributed efficiently to clinical areas, ensuring they remain sterile until use.

Adherence to standards from organizations like the Association for the Advancement of Medical Instrumentation (AAMI), the Centers for Disease Control and Prevention (CDC), and regulatory bodies such as the FDA is essential. Trained sterile processing technicians manage this workflow, using quality control measures like biological indicators and routine audits to uphold efficacy and compliance.

Table of contents

Part 1: OnlineExamMaker – Generate and share sterile processing quiz with AI automatically

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Part 2: 20 sterile processing quiz questions & answers

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1. Question: What is the primary goal of sterile processing in a healthcare setting?
A) To enhance the aesthetic appeal of medical instruments
B) To eliminate microorganisms and prevent infections
C) To increase the weight of surgical tools
D) To speed up patient discharge

Answer: B) To eliminate microorganisms and prevent infections

Explanation: Sterile processing ensures that medical instruments are free from pathogens, reducing the risk of healthcare-associated infections.

2. Question: Which method uses high-pressure steam for sterilization?
A) Chemical disinfection
B) Autoclaving
C) Dry heat sterilization
D) Ultraviolet light

Answer: B) Autoclaving

Explanation: Autoclaving employs saturated steam under pressure to kill microorganisms by denaturing proteins.

3. Question: What is the minimum exposure time for steam sterilization at 121°C?
A) 5 minutes
B) 15 minutes
C) 30 minutes
D) 60 minutes

Answer: B) 15 minutes

Explanation: At 121°C, a standard cycle requires at least 15 minutes of exposure to achieve sterilization, depending on load and packaging.

4. Question: Which indicator verifies that sterilization conditions were met inside a pack?
A) Chemical indicator
B) Biological indicator
C) Temperature gauge
D) pH strip

Answer: B) Biological indicator

Explanation: Biological indicators, such as spore strips, confirm sterilization by testing for the survival of resistant microorganisms.

5. Question: What is the purpose of wrapping instruments before sterilization?
A) To make them easier to transport
B) To maintain sterility until use
C) To reduce the instrument’s weight
D) To change their color

Answer: B) To maintain sterility until use

Explanation: Wrapping with appropriate materials creates a barrier that protects against contamination post-sterilization.

6. Question: In sterile processing, what does “event-related sterility” mean?
A) Sterility based on the date of processing
B) Sterility maintained until an event compromises it
C) Sterility related to patient events
D) Sterility checked every hour

Answer: B) Sterility maintained until an event compromises it

Explanation: Event-related sterility holds that items remain sterile until they are exposed to conditions that could cause contamination.

7. Question: Which personal protective equipment (PPE) is essential during decontamination?
A) Surgical gown only
B) Gloves, mask, and eye protection
C) Shoes and hat
D) Apron alone

Answer: B) Gloves, mask, and eye protection

Explanation: PPE like gloves, masks, and eye protection prevents exposure to biohazards during the decontamination process.

8. Question: What is the first step in the sterile processing workflow?
A) Sterilization
B) Decontamination
C) Packaging
D) Storage

Answer: B) Decontamination

Explanation: Decontamination removes soil and microorganisms from instruments before further processing to ensure safety.

9. Question: Which chemical is commonly used for high-level disinfection?
A) Bleach
B) Glutaraldehyde
C) Soap
D) Alcohol

Answer: B) Glutaraldehyde

Explanation: Glutaraldehyde is effective for high-level disinfection of heat-sensitive instruments by killing most microorganisms.

10. Question: How should sterile items be stored to maintain sterility?
A) In open shelves
B) In dust-covered areas
C) In clean, dry, closed cabinets
D) On the floor

Answer: C) In clean, dry, closed cabinets

Explanation: Proper storage in closed cabinets protects items from environmental contaminants, preserving sterility.

11. Question: What does AAMI stand for in sterile processing standards?
A) Association for Medical Instrument Inspection
B) American Association of Medical Instrumentation
C) Advanced Automated Medical Integration
D) Association of American Medical Institutions

Answer: B) American Association of Medical Instrumentation

Explanation: AAMI provides guidelines and standards for the processing of medical devices to ensure quality and safety.

12. Question: Which factor is critical for effective ethylene oxide sterilization?
A) High humidity
B) Low temperature only
C) Absence of aeration
D) Direct sunlight

Answer: A) High humidity

Explanation: Ethylene oxide requires controlled humidity to penetrate and sterilize items effectively.

13. Question: What is the role of a sterile processing technician?
A) To perform surgeries
B) To clean and sterilize instruments
C) To administer medications
D) To manage patient records

Answer: B) To clean and sterilize instruments

Explanation: Technicians are responsible for the entire cycle of instrument reprocessing to support safe medical procedures.

14. Question: Why are biological monitors used in sterilization?
A) To check packaging integrity
B) To confirm microbial kill
C) To measure temperature
D) To clean surfaces

Answer: B) To confirm microbial kill

Explanation: Biological monitors test the efficacy of sterilization by detecting if resistant spores have been destroyed.

15. Question: What is the typical temperature for dry heat sterilization?
A) 100°C
B) 121°C
C) 160°C
D) 200°C

Answer: C) 160°C

Explanation: Dry heat sterilization at around 160°C for a specified time ensures destruction of microorganisms through oxidation.

16. Question: Which practice helps prevent cross-contamination in sterile processing?
A) Reusing single-use items
B) Proper hand hygiene and workflow separation
C) Storing items in humid areas
D) Sharing instruments between patients

Answer: B) Proper hand hygiene and workflow separation

Explanation: Separating clean and dirty areas, along with hand hygiene, minimizes the risk of cross-contamination.

17. Question: What should be done if a sterilization cycle fails?
A) Use the items anyway
B) Reprocess the items immediately
C) Store them for later use
D) Discard without investigation

Answer: B) Reprocess the items immediately

Explanation: Failed cycles require reprocessing to ensure items meet sterility standards before use.

18. Question: Which type of packaging is suitable for steam sterilization?
A) Plastic bags
B) Paper-plastic pouches
C) Aluminum foil
D) Cloth wraps

Answer: B) Paper-plastic pouches

Explanation: Paper-plastic pouches allow steam penetration while maintaining a sterile barrier after the cycle.

19. Question: How often should sterile processing equipment be maintained?
A) Annually
B) According to manufacturer recommendations
C) Never
D) Only when it breaks

Answer: B) According to manufacturer recommendations

Explanation: Regular maintenance as per guidelines ensures equipment reliability and prevents sterilization failures.

20. Question: What is the main risk of improper sterile processing?
A) Increased costs
B) Healthcare-associated infections
C) Faster instrument wear
D) Delayed procedures

Answer: B) Healthcare-associated infections

Explanation: Inadequate processing can lead to contaminated instruments, increasing the incidence of infections in patients.

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