Biological Engineering, also known as bioengineering, is a multidisciplinary field that applies engineering principles to biological systems and living organisms. It combines biology, chemistry, physics, and engineering to solve problems in areas such as healthcare, agriculture, and environmental sustainability.
At its core, biological engineering focuses on designing, developing, and optimizing processes involving biological materials. This includes manipulating genetic code, creating biomaterials, and engineering cellular systems for practical applications.
Key subfields include:
– Genetic Engineering: Modifying DNA in organisms to enhance traits, such as developing disease-resistant crops or gene therapies for humans.
– Biomedical Engineering: Designing medical devices, prosthetics, and tissue scaffolds to improve diagnostics, treatments, and patient outcomes.
– Bioprocess Engineering: Scaling up biological processes for industrial production, like manufacturing biofuels, pharmaceuticals, and enzymes.
– Environmental Biotechnology: Using microorganisms for waste treatment, pollution control, and sustainable resource management.
Applications of biological engineering are wide-ranging. In medicine, it enables advancements in regenerative medicine, personalized medicine, and implantable devices. In agriculture, it improves crop yields, reduces pesticide use, and supports food security. Environmentally, it contributes to bioremediation, renewable energy sources, and climate change mitigation.
The field integrates tools like CRISPR for precise gene editing, synthetic biology for creating novel organisms, and bioinformatics for data analysis. As technology evolves, biological engineering continues to address global challenges, from pandemics to food shortages, driving innovation in science and industry.
Table of Contents
- Part 1: OnlineExamMaker – Generate and Share Biological Engineering Quiz with AI Automatically
- Part 2: 20 Biological Engineering Quiz Questions & Answers
- Part 3: AI Question Generator – Automatically Create Questions for Your Next Assessment

Part 1: OnlineExamMaker – Generate and Share Biological Engineering Quiz with AI Automatically
The quickest way to assess the Biological Engineering knowledge of candidates is using an AI assessment platform like OnlineExamMaker. With OnlineExamMaker AI Question Generator, you are able to input content—like text, documents, or topics—and then automatically generate questions in various formats (multiple-choice, true/false, short answer). Its AI Exam Grader can automatically grade the exam and generate insightful reports after your candidate submit the assessment.
What you will like:
● Create a question pool through the question bank and specify how many questions you want to be randomly selected among these questions.
● Allow the quiz taker to answer by uploading video or a Word document, adding an image, and recording an audio file.
● Display the feedback for correct or incorrect answers instantly after a question is answered.
● Create a lead generation form to collect an exam taker’s information, such as email, mobile phone, work title, company profile and so on.
Automatically generate questions using AI
Part 2: 20 Biological Engineering Quiz Questions & Answers
or
1. What is the primary function of restriction enzymes in genetic engineering?
A. To amplify DNA sequences
B. To cut DNA at specific sequences
C. To join DNA fragments
D. To replicate DNA
Answer: B
Explanation: Restriction enzymes act as molecular scissors that recognize and cut DNA at specific nucleotide sequences, enabling the insertion or removal of genes in genetic engineering.
2. In bioprocess engineering, what does a bioreactor primarily facilitate?
A. Chemical synthesis of drugs
B. Controlled environment for cell growth and product formation
C. Purification of proteins
D. Storage of biological samples
Answer: B
Explanation: A bioreactor provides an optimal environment for microbial, plant, or animal cells to grow and produce desired products, such as antibiotics or biofuels, by controlling factors like pH, temperature, and nutrients.
3. Which technique is used to introduce foreign DNA into plant cells?
A. Polymerase chain reaction (PCR)
B. Agrobacterium-mediated transformation
C. Gel electrophoresis
D. Western blotting
Answer: B
Explanation: Agrobacterium-mediated transformation uses the natural ability of Agrobacterium tumefaciens to transfer T-DNA into plant genomes, making it a key method for creating genetically modified crops.
4. What is the role of plasmids in recombinant DNA technology?
A. They serve as vectors to carry foreign genes into host cells
B. They inhibit bacterial growth
C. They code for ribosomal RNA
D. They are used for DNA sequencing
Answer: A
Explanation: Plasmids are small, circular DNA molecules that can be engineered to carry and express foreign genes in host organisms, such as bacteria, facilitating cloning and protein production.
5. In tissue engineering, what is the purpose of a scaffold?
A. To provide structural support for cell growth and tissue formation
B. To deliver drugs directly to tissues
C. To measure cellular activity
D. To store biomaterials
Answer: A
Explanation: Scaffolds act as a three-dimensional framework that supports cell attachment, proliferation, and differentiation, mimicking the extracellular matrix to regenerate tissues like skin or bone.
6. What does CRISPR-Cas9 primarily enable in biological engineering?
A. Random mutation of genes
B. Precise editing of DNA sequences
C. Amplification of RNA
D. Protein purification
Answer: B
Explanation: CRISPR-Cas9 is a genome-editing tool that uses guide RNA to direct the Cas9 enzyme to cut specific DNA sequences, allowing for targeted insertions, deletions, or modifications.
7. Which process is essential for producing biofuels from microorganisms?
A. Fermentation
B. Photosynthesis
C. Transpiration
D. Osmosis
Answer: A
Explanation: Fermentation allows microorganisms to convert sugars into biofuels like ethanol by anaerobic metabolism, making it a core process in bioenergy production.
8. What is metabolic engineering primarily concerned with?
A. Modifying cellular metabolic pathways to enhance product yield
B. Studying animal behavior
C. Designing medical implants
D. Analyzing environmental pollutants
Answer: A
Explanation: Metabolic engineering involves redesigning metabolic networks in organisms, such as bacteria, to overproduce valuable compounds like pharmaceuticals or chemicals.
9. In biological engineering, what is the function of biomaterials?
A. To interact with biological systems for medical applications
B. To conduct electrical signals
C. To filter water
D. To generate energy
Answer: A
Explanation: Biomaterials are designed to be compatible with living tissues, serving purposes like implants or drug delivery, by mimicking natural properties to promote healing or function.
10. Which method is used to separate proteins based on their size?
A. SDS-PAGE
B. ELISA
C. PCR
D. Mass spectrometry
Answer: A
Explanation: SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) denatures proteins and separates them by molecular weight, allowing for analysis in protein engineering.
11. What is the main advantage of using stem cells in regenerative medicine?
A. Their ability to differentiate into various cell types
B. Their resistance to diseases
C. Their rapid division rate
D. Their storage stability
Answer: A
Explanation: Stem cells can be directed to become specific cell types, such as neurons or muscle cells, enabling the repair or replacement of damaged tissues in regenerative therapies.
12. In environmental biotechnology, what is bioremediation?
A. Using microorganisms to degrade pollutants
B. Creating synthetic ecosystems
C. Generating electricity from waste
D. Storing carbon dioxide
Answer: A
Explanation: Bioremediation employs microbes to break down environmental contaminants, such as oil spills, into less harmful substances, restoring ecosystems.
13. What is synthetic biology’s primary goal?
A. Designing new biological parts and systems for specific functions
B. Studying natural evolution
C. Preserving endangered species
D. Analyzing fossil records
Answer: A
Explanation: Synthetic biology involves engineering biological components, like genes or circuits, to create novel organisms or products, such as biosensors or biofuels.
14. Which enzyme is commonly used in PCR for DNA amplification?
A. Taq polymerase
B. Restriction endonuclease
C. Ligase
D. Reverse transcriptase
Answer: A
Explanation: Taq polymerase is a heat-stable enzyme that synthesizes DNA during the PCR process, allowing for repeated cycles of amplification without degradation.
15. In drug delivery systems, what is a liposome?
A. A vesicle that encapsulates drugs for targeted delivery
B. A protein that binds to receptors
C. A synthetic hormone
D. A bacterial toxin
Answer: A
Explanation: Liposomes are spherical vesicles made of lipids that can carry drugs to specific sites in the body, improving efficacy and reducing side effects.
16. What is the purpose of biosensors in biological engineering?
A. To detect biological molecules or processes in real-time
B. To store genetic data
C. To synthesize antibodies
D. To culture cells
Answer: A
Explanation: Biosensors use biological components, like enzymes or antibodies, integrated with transducers to monitor analytes, such as glucose levels, for medical or environmental applications.
17. Which technique is used to sequence DNA in genomics?
A. Next-generation sequencing
B. X-ray crystallography
C. Chromatography
D. Centrifugation
Answer: A
Explanation: Next-generation sequencing technologies rapidly determine the order of nucleotides in DNA, enabling applications in genetic engineering and personalized medicine.
18. In protein engineering, what is site-directed mutagenesis?
A. Introducing specific changes to a protein’s amino acid sequence
B. Random protein folding
C. Protein degradation
D. Antibody production
Answer: A
Explanation: Site-directed mutagenesis alters specific nucleotides in a gene to change the resulting protein’s function, structure, or stability for applications like enzyme optimization.
19. What is the ethical concern associated with genetically modified organisms (GMOs)?
A. Potential environmental impact and gene transfer to wild populations
B. Increased nutritional value
C. Faster crop growth
D. Reduced pesticide use
Answer: A
Explanation: GMOs raise ethical issues due to risks like unintended ecological consequences or cross-breeding with non-GMO species, prompting regulations in biological engineering.
20. In nanobiotechnology, what are quantum dots used for?
A. Imaging and labeling biological molecules at the nanoscale
B. Energy production
C. Drug metabolism
D. Waste disposal
Answer: A
Explanation: Quantum dots are semiconductor nanoparticles that emit light when excited, making them useful for tracking cells or molecules in biological imaging and diagnostics.
or
Part 3: AI Question Generator – Automatically Create Questions for Your Next Assessment
Automatically generate questions using AI